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Histology PDF Print E-mail

 
Director
Dr. Thomas Norton
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Manager
Tammy Bailey
Worrell Building Room 310
Phone: (205) 934-3074
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Services

The primary purpose of the Histology module is to assist the 56 members of the Vision Science Research Center to complete histology-related portions of their research. This is accomplished by meeting with the researcher to define the approach to the problem, developing or modifying procedures needed, and completing the project as outlined. The histology module also exists to provide expertise for training and supervision of graduate students and personnel from other labs in histology procedures and in the use of available laboratory equipment; for solving technical problems for these personnel; and to purchase sufficient equipment and supplies for an efficiently operating lab.

 

About

 

The Histology Analysis Module is an 800 square feet facility on the 3rd floor of the Worrell Building. The laboratory is divided into 3 main rooms designed for specific functions

  • Room 310A (247 square feet) - cryostat and semi-thin microtomy, glass knifemaking, sink, ice machine, and hood and balance for hazardous substances.
  • Room 310B (282 square feet) - hood for perfusions and general purposes, staining, Vibratome and sledge microtome sectioning, downdraft countertops, dishwasher, darkroom, and Corning distiller for deionized/distilled water
  • Room 310C (270 square feet) - research assistant/manager's desk, photo-and video-microscopy, 3 computers for analysis, etc.
  • Room 508 (93.5 square feet) - Additional space on the 5th floor of the Worrell Building houses freezers and an autoclave.



Other


Ultracold freezers and an antoclave are located on the 5th floor (Rm. 508) of the Worrell Building. An additional ultracold freezer is on the 3rd floor of the Peters Optometry Building.

 

MICROSCOPY

  • Leitz Aristoplan microscope with epifluorescence (5 cubes), DIC, bright- and dark-field capabilities and an XYZ-encoded stage.






  • Leitz Orthoplan microscope with epifluorescence, bright- and dark-field capabilities and a drawing tube option.



  •  



  • Wild Photomakroskop with bright- and dark-field.
  • Zeiss scopes - two, a Standard and a wet lab scope.
  • Bausch & Lomb projecting microscope.
  • Nikon Disecting Microscope with plastic adaptable trimming head.


 

 

VIDEOMICROSCOPY

  • SONY DXC-906 MC/LLP is a 3-chip CCD camera for acquiring brightfield or fluorescent images. There is a frame-averaging capability for low-light fluorescence. (Used with the Aristoplan scope for the NeuroLucida morphometry program.)
  • SONY DXC-151 is a single-chip CCD camera for brightfield images. (Used to make video prints from the Orthplan scope.)
  • Diagnostic Instruments' cooled CCD, color, digital SPOT camera for high quality brightfield or low light level specimens--fluorescence or dark field. (Used with a KODAK 8650 PS dye-sublimation printer for photographic quality prints.)
  • SONY UP-5000 video printer for instant prints from the Aristoplan or Orthoplan microscopes.

    PHOTOMICROSCOPY (35 mm)

    • Wild MPS-46 Photoautomat and Wild MPS-52
      camera.
    • Olympus C-35A camera system
    • Wild MPS-55 photoautomat on the Wild scope.
    • Dark room used for film development.
     

    MICROTOMY & ACCESSORIES

    • Reichert-Jung 2800-E cryostat (thin, fix or unfixed sections)
    • Jung 2065 Supercut and Sorvall J-4 microtomes (both for GMA, soft plastic, semi-thin sections)


    • AO sliding microtome (celloidin & frozen sections)
    • AO rotary microtome (paraffin)
    • Paraffin dispenser, slide warming trays, tissue flotation baths
    • Lancer 1000 Vibratome (thicker fix or unfixed sections)


    • Glass knifemakers: LKB & Energy Beam Sciences for Ralph knives
    • LKB triangle knifemaker
    • AO Knife sharpener for steel knives
    • Lipshaw Section Freeze
  • GENERAL LABORATORY EQUIPMENT

    • Mettler balances, 3 (one dedicated to hazardous materials)



    • Labconco dishwasher with deionized water final rinse and/or acid rinse options.



    • High flow hoods, 2 (one with equipment for perfusions)



    • Ice machines, 2 (crushed and mini-cubes)
    • Ovens, 3 (electric, convection, vacuum)
    • pH meters, 3 Refrigerators, 3 large & 3 small (2 dedicated to radioactive substances, 1 for autoradiography exposure)
    • Corning Mega-Pure distiller and collections system (for deionized, glass-distilled water)



    • Finnpipets, peristaltic and vacuum pumps, shaker table, stirrer/hotplates.
Last Updated ( Wednesday, 17 September 2008 )
 
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